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Membranes were washed three times in 1PBS and positive signals were developed with enhanced chemiluminescence kit (Amershan Pharmacia Biotech, Little Chalfont Buckinghamshire, England)

As we age, mitochondrial function declines, leading to increased oxidative stress, reduced ATP production, energy deficiency, and the accumulation of cellular damage over time

[DOI] [PubMed] [Google Scholar] 33.Fruhbeck G, Aguado M, Martinez JA